가토 두개골 결손 모델에서 실크단백과 나노하이드록시아파타이트, 옥수수 녹말 복합물을 이용한 골 이식재 개발
The Effect of Silk Fibroin/Nano-hydroxyapatite/Corn Starch Composite Porous Scaffold on Bone Regeneration in the Rabbit Calvarial Defect Model
박용태, 권광준, 박영욱, 김성곤, 김찬우, 조유영, 권해용, 강석우,
소속 상세정보
박용태 ( Park Yong-Tae ) - 강릉원주대학교 치과대학 구강악안면외과학교실
권광준 ( Kwon Kwang-Jun ) - 강릉원주대학교 치과대학 구강악안면외과학교실
박영욱 ( Park Young-Wook ) - 강릉원주대학교 치과대학 구강악안면외과학교실
김성곤 ( Kim Seong-Gon ) - 강릉원주대학교 치과대학 구강악안면외과학교실
김찬우 ( Kim Chan-Woo ) - 강릉원주대학교 치과대학 구강악안면외과학교실
조유영 ( Jo You-Young ) - 농촌진흥청
권해용 ( Kweon Hae-Yong ) - 농촌진흥청
강석우 ( Kang Seok-Woo ) - 농촌진흥청
KMID : 0360120110330060459
Abstract
Purpose: This study evaluated the capability of bone formation with silk fibroin/nano-hydroxyapatite/corn starch composite scaffold as a bone defect replacement matrix when grafted in a calvarial bone defect of rabbits in vivo.
Methods: Ten New Zealand white rabbits were used for this study and bilateral round-shaped defects were formed in the parietal bone (diameter: 8.0 mm). The silk fibroin 10% nano-hydroxyapatite/30% corn starch/60% composite scaffold was grafted into the right parietal bone (experimental group). The left side (control group) was grafted with a nano-hydroxyapatite (30%)/corn starch (70%) scaffold. The animals were sacrificed at 4 weeks and 8 weeks. A micro-computerized tomography (microCT) of each specimen was taken. Subsequently, the specimens were decalcified and stained with Masson’s trichrome for histological and histomorphometric analysis.
Results: The average microCT and histomorphometric measures of bone formation were higher in the control group than in the experimental group at 4 weeks and 8 weeks after surgery though not statistically significant (P>0.05).
Conclusion: The rabbit calvarial defect was not successfully repaired by silk fibroin/nano-hydroxyapatite/corn starch composite scaffold and may have been due to an inflammatory reaction caused by silk powder. In the future, the development of composite bone graft material based on various components should be performed with caution.
키워드
Silk fibroin/nano-hydroxyapatite/corn starch composite scaffold; Micro-computed tomography; Bone regeneration
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